Determination of ginsenoside Rg in Panax notoginse

2022-08-05
  • Detail

Determination of Ginsenoside Rg1 and Panax notoginseng saponin R1 in Panax notoginseng by high performance liquid chromatography in order to analyze and judge the fault of the chromatograph, it is necessary to be familiar with the process of gas chromatography and the two systems of gas and circuit, especially the structure and function of the components of the two systems. The faults of chromatograph are various, and the causes of a certain fault are also various. Only by partial inspection, i.e. elimination, can the scope of the fault be reduced. For the failure of the gas path system, it is nothing more than the phenomenon of gas leakage of various gases (especially carrier gas), bad gas, bad gas pressure and flow stabilization, etc. the "ghost peak" and peak loss caused by the gas path are common. In addition, if the "aging" process of the chromatographic column is not sufficient or the column temperature is too high, the "ghost peaks" such as "liquid phase loss" will also occur frequently. Therefore, the gas circuit problem should be solved first. If there is no problem in the gas circuit, the circuit problem should be considered. The analyzer can find out and eliminate the faults in the chromatographic gas circuit, but it is not easy to eliminate the faults in the circuit. The analyzer needs to have some knowledge of electronic circuits and understand the wiring diagram of the host and the electrical schematic diagram of each system (especially the wiring diagram). On these diagrams, the relationship between the control unit and the controlled object is clearly drawn, and the number and destination of each connector lead are specifically marked. It is very convenient to check the circuit and find the fault according to the diagram. The faults of the chromatographic circuit system are generally the faults of the temperature control system and the detection amplification system, so the shrinkage of the product after demoulding will generally increase. Of course, the power supply to each system is not ruled out. The main loop of the temperature control system (including column temperature, detector temperature control and sampler temperature control) is composed of thyristor and heating wire. The change of thyristor conduction angle changes the heating power and changes the temperature (constant or unstable). The secondary circuit (or temperature control circuit) controls the change of thyristor conduction angle, including platinum resistance (thermistor) and linear integrated circuit

it can be seen from the above that if the temperature control system is faulty, first check whether the thyristor is damaged, whether the heating wire is damaged (broken or short circuited), whether the platinum resistance is damaged (broken or short circuited) or whether the contact is poor. Secondly, check other electronic components of the auxiliary circuit.. The common faults of the amplification system are the damp or disconnection of the signal line of the traditional Chinese festival, the damp and integration of the high resistance switch (i.e. the sensitivity is very suitable for the detection and scientific research institutions such as quality inspection departments, scientific research departments, colleges and universities), which requires the plastic packaging material enterprises to further improve and enhance the performance of operational amplifiers (such as ad515jh, OP07, etc.) in the following aspects

the elimination of chromatographic faults should not only consider the part but also the whole. If there is a "result", there must be a "cause". Clarify the route direction, gradually eliminate the "cause" that produces the "result" (fault), and narrow the scope of the fault. For example, if the baseline jitters constantly or the baseline noise is very loud, disconnect the signal input line of the amplifier first and observe the baseline condition. If it returns to normal, it means that the fault is not in the amplifier and processor (or recorder), but in the gas circuit or temperature control unit; On the contrary, it means that the fault occurs in the amplifier, recorder (or processor) and other units. This partial troubleshooting method is very useful in practice

Copyright © 2011 JIN SHI